A New Pseudomonas aeruginosa Isolate Enhances Its Unusual 1,3-Propanediol Generation from Glycerol in Bioelectrochemical System

The ability of some bacteria to perform Extracellular Electron Transfer (EET) has been explored in bioelectrochemical systems (BES) obtain energy or chemicals from pure substances residual substrates. Here, a new pyoverdine-producing Pseudomonas aeruginosa strain was isolated an MFC biofilm oxidizing glycerol, by-product biodiesel production. Strain EL14 investigated assess its electrogenic and products. In open circuit system (fermentation system), able consume glycerol produce 1,3-propanediol, unusual product oxidation P. aeruginosa. microbial fuel cell (MFC) reached current density 82.4 mA m−2 during the first feeding cycle, then dropped sharply as fell off. Cyclic voltammetry suggests that electron transfer anode occurs indirectly, i.e., through redox substance, with peak at 0.22 V (vs Ag/AgCl), directly probably by membrane proteins, 0.05 Ag/AgCl). produced added-value bioproducts, acetic butyric acids, well 1,3 propanediol, both fermentative anodic conditions. However, yield 1,3-PDO enhanced 0.57 0.89 (mol mol−1 glycerol) under conditions compared fermentation. This result unexpected, since successful production is not usually associated metabolism. By comparing genomic sequences related biosynthesis reference strains, we observed three copies dhaT gene (1,3-propanediol dehydrogenase different arrangement other isolates). Thus, this work functionally characterizes bacterium never before biosynthesis, indicating potential for converting industry into emerging chemical product.